DNA evidence used to protect the rhinoceros from extinction

Rhinoceros.

Call it CSI meets conservation. Stephen O'Brien, Ph.D., a research scientist at Nova Southeastern University's (NSU) Halmos College of Natural Sciences and Oceanography, has worked with colleagues across the globe to create a DNA database of rhinoceros that will be used to help with the criminal prosecution of poachers.

"What we've done is create a powerful tool -- a genetic database -- that enforcement officials can use when they are building cases against those accused of poaching," Dr. O'Brien said. "These magnificent animals are facing increased dangers, so we need to bring every resource we can to the efforts to save them for generations to come."

The research team included experts from the University of Pretoria's Veterinary Genetics Laboratory, the Theodosius Dobzhansky Center for Genome Bioinformatics, St. Petersburg State University and African wildlife enforcement units.

A new report addresses the illegal taking of African rhinoceros -- an important conservation icon -- by organized crime syndicates trafficking rhino horns to southeast Asia markets. Rhino poaching is uncontrolled and increasing, from a few incidents a decade ago to more than 1,000 per year today. The profits are enormous, and so far the risks rather modest.

This study changes that.

The study, published today in Current Biology titled "Robust forensic matching of confiscated horns to individual poached African rhinoceros," describes a comprehensive effort to create a large database of individual rhinos' composite short-term repeat-STR (also called microsatellite) genotypes so they could match confiscated tissue-DNA to real time crime scenes for prosecution.

The team developed an extensive database of rhinoceros DNA profiles and demographic information named RhODIS® (Rhino DNA Index System), modeled after CODIS, which is the U.S. FBI's criminal DNA database. The RhODIS® forensic system involved African wildlife ranger training and certification, a chain of custody compliant sampling methodology used for live and dead rhinoceros and rhinoceros horns, an eRhODIS™ field data collection "app" and state-of-the-art DNA genetic individualization. To date, more than 20,000 individual rhinoceros specimens and genotypes have been added to the RhODIS® database.

These data include more than 5,800 forensic case samples where links were made between recovered horns, blood stained evidence items and specific rhinoceros carcasses. In the most recent cases listed in the report, this forensic genetic individualization allowed heavy punishments upon conviction, establishing international legal precedents for prosecuting and convicting smugglers of rhino horns suitable for other endangered species traffic. The applications highlight the legal precedent of utilizing the RhODIS® system for rhinoceros crimes and provide a benchmark for implementation of similar systems for global wildlife trafficking investigations of other species.

African black and white rhinoceros (Diceros bicornis and Ceratotherium simum) are classified on as critically endangered and near threatened, respectively. Their continued survival is jeopardized by habitat loss and a surge in illegal hunting for their horns that are considered of both medicinal value and as a cultural status symbol in Asian countries, mainly Vietnam and China. More than 7,000 rhinoceros have been killed through poaching in the past decade across Africa with South Africa suffering the highest losses.

"We've seen a huge surge in the number of rhinos poached over the past couple of years simply because the financial reward far outweighed the risk," Dr. O'Brien said. "Now that we can genetically tie someone to a specific cases of poaching, the chances of successful prosecution will go up -- hopefully the risk will now not be worth it."

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