The first Neolithic boats in the Mediterranean

More than 7,000 years ago, people navigated the Mediterranean Sea using technologically sophisticated boats, according to a study published March 20, 2024 in the open-access journal PLOS ONE by Juan F. Gibaja of the Spanish National Research Council, Barcelona and colleagues.

Many of the most important civilizations in Europe originated on the shores of the Mediterranean Sea.

During the Neolithic, communities clearly traveled and traded across the water, as evidenced by watercraft in the archeological record and the presence of settlements on coasts and islands.

In this study, Gibaja and colleagues provide new insights into the history of seafaring technology through analysis of canoes at the Neolithic lakeshore village of La Marmotta, near Rome, Italy.

Excavation at this site has recovered five canoes built from hollowed-out trees (dugout canoes) dating between 5700-5100BC.

Analysis of these boats reveals that they are built from four different types of wood, unusual among similar sites, and that they include advanced construction techniques such as transverse reinforcements.

One canoe is also associated with three T-shaped wooden objects, each with a series of holes that were likely used to fasten ropes tied to sails or other nautical elements.

These features, along with previous reconstruction experiments, indicate these were seaworthy vessels, a conclusion supported by the presence at the site of stone tools linked to nearby islands.

The authors describe these canoes as exceptional examples of prehistoric boats whose construction required a detailed understanding of structural design and wood properties in addition to well-organized specialized labor.

Similarities between these canoes and more recent nautical technologies support the idea that many major advances in sailing were made during the early Neolithic.

The authors suggest there may be more boats preserved near La Marmotta, a potential avenue for future research.

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Neolithic ceramics reveal dairy processing from milk of multiple species

A new study has found evidence of cheesemaking, using milk from multiple animals in Late Neolithic Poland.

The research suggests that early farmers reduced the lactose content in milk by making it into cheese or other dairy products like yoghurt, and used dairy products from a number of different animals, such as cows, sheep or goats.

Lactose intolerance was a common condition in almost everyone in Europe during the Neolithic and until the Late Bronze Age when the genetic mutation became widespread, enabling adults to produce lactase, the enzyme which breaks down lactose in the body.

Researchers looked at the practice of dairy processing in the Late Neolithic, identifying high curd-content residues in pottery indicating cheesemaking, and revealing that multiple dairy species were utilised.

Dr Harry Robson, from the Department of Archaeology at the University of York, said: "These results contribute significantly to our understanding of the use of dairy products by some of the earliest farmers of Central Europe.

"Whilst previous research has shown that dairy products were widely available in some European regions during this period, here, for the first time, we have clear evidence for a diversified dairy herd, including cattle, sheep and goats, from the analysis of ceramics."

The scientists and archaeologists from the Universities of York, Cambridge, Toru? and Kraków used a multi-stranded proteomic and lipid-analysis approach to investigate ceramics and deposits on their surface, from the site of S?aw?cinek in central Poland.

The new development provides evidence that cheesemaking (and other curd-enriching dairy processing) can be directly detected by scrutinising the proportion of curd proteins, by comparing proteomic data. The results are also the first of their kind in Europe.

Despite widespread lactose intolerance in the period, there is evidence of dairy being consumed during the Neolithic, such as animal bones with kill patterns expected for dairy herds, dairy lipids in ceramic vessels, and dairy proteins in ancient dental calculus or plaque.

Lead author, Miranda Evans, PhD student at Cambridge's Department of Archaeology, said: "The proteomic results showed that the ancient residues closely resembled both the modern cheesemaking residues and cheese itself and not whole milk. This reveals that the people of S?aw?cinek practised cheesemaking or another form of curd-enriching dairy processing."

Evidence of multiple species used for cheesemaking was backed up by the presence of both cow and sheep or goat bones on the site."

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New analysis of obsidian blades reveals dynamic Neolithic social networks

An analysis of obsidian artifacts excavated during the 1960s at two prominent archaeological sites in southwestern Iran suggests that the networks Neolithic people formed in the region as they developed agriculture are larger and more complex than previously believed, according to a new study by Yale researchers.

The study, published Oct. 17 in the journal Proceedings of the National Academy of Sciences, is the first to apply state-of-the-art analytical tools to a collection of 2,100 obsidian artifacts housed at the Yale Peabody Museum. The artifacts were unearthed more than 50 years ago at Ali Kosh and Chagha Sefid, sites on Iran's Deh Luran Plain that yielded important archaeological discoveries from the Neolithic Era -- the period beginning about 12,000 years ago when people began farming, domesticating animals, and establishing permanent settlements.

Original analyses performed shortly after the artifacts were discovered had suggested people first acquired the obsidian -- volcanic glass -- from Nemrut Dağ, a now dormant volcano in Eastern Turkey, and then relied on an unknown second source for the material. This new elemental analysis showed the obsidian came from seven distinct sources, including Nemrut Dağ, in present-day Turkey and Armenia, which is as far as about 1,000 miles on foot from the excavation sites.

"It wasn't a simple pattern of people obtaining obsidian from one source and then shifting to the next," said Ellery Frahm, an archaeological scientist in the Department of Anthropology in Yale's Faculty of Arts and Sciences, and the study's lead author. "Rather, our analysis shows that they were acquiring obsidian from an increasingly diverse number of geological sources over time -- a trend that was impossible to detect with the technology and methods available 50 years ago."

The new analysis, combined with computer modeling, indicates that there were intensifying connections among Neolithic people, suggesting the presence of a greater number of settlements between the source volcanoes and the two sites where the artifacts were unearthed thousands of years later, Frahm said.

The artifacts were collected in the 1960s during multiple excavations of the two sites led by Frank Hole, the C.J. MacCurdy Professor Emeritus of Anthropology at Yale. The initial analyses were based largely on the artifacts' appearance, specifically their color when held up to sunlight. A subset of 28 artifacts were then subjected to an elemental analysis method common at the time that involved grinding them into powder.

Frahm and coauthor Christina M. Carolus, a doctoral student in the Department of Anthropology, are the first researchers to study the elemental composition of the obsidian artifacts since these early analyses. They used state-of-the-art portable X-ray fluorescence instruments, which allowed them to examine the entire collection without damaging the artifacts.

"Every aspect of the discoveries made at these sites had been revisited since the 1960s except the elemental composition and sourcing of the obsidian artifacts," Carolus said. "A lot more is known about the source volcanoes today than 50 years ago, and we know that sorting obsidian by color will miss a lot of nuances. Fortunately, we have instruments the size of cordless drills that, in a matter of seconds and without destroying material, give us a more accurate elemental signature than anything that was possible in the past."

Scientists widely believed that humanity's transition from the hunter-gatherer lifestyle to agriculture produced a period of rapid population growth due to the increased birth rates made possible by enhanced food supplies and permanent settlements. Finding evidence of this demographic shift often requires excavating locations that include burial sites, which can indicate a given settlement's population and provide a clearer picture of how agriculture allowed people to disperse across a landscape, Frahm said.

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Genetic histories and social organization in Neolithic and Bronze Age Croatia

Present-day Croatia was an important crossroads for migrating peoples along the Danubian corridor and the Adriatic coast, linking east and west. "While this region is important for understanding population and cultural transitions in Europe, limited availability of human remains means that in-depth knowledge about the genetic ancestry and social complexity of prehistoric populations here remains sparse," says first author Suzanne Freilich, a researcher at the Max Planck Institute for the Science of Human History and the University of Vienna.

To this aim, an international team of researchers set out to fill the gap. They studied two archaeological sites in eastern Croatia -- one containing predominantly Middle Neolithic burials from within the settlement site, the other a Middle Bronze Age necropolis containing cremations and inhumations -- and sequenced whole genomes of 28 individuals from these two sites. The researchers' goal was to understand both the genetic ancestry as well as social organisation within each community -- in particular, to study local residency patterns, kinship relations and to learn more about the varied burial rites observed.

Middle Neolithic settlement at Popova zemlja

Dated to around 4,700-4,300 BCE the Middle Neolithic settlement at Beli-Manastir Popova zemlja belongs to the Sopot culture. Many children, especially girls, were buried here, in particular along the walls of pit houses. "One question was whether individuals buried in the same buildings were biologically related to each other," says Suzanne Freilich.

"We found that individuals with different burial rites did not differ in their genetic ancestry, which was similar to Early Neolithic people. We also found a high degree of haplotype diversity and, despite the size of the site, no very closely related individuals," Freilich adds. This suggests that this community was part of a large, mainly exogamous population where people marry outside their kin group. Interestingly, however, the researchers also identified a few cases of endogamous mating practices, including two individuals who would have been the children of first cousins or equivalent, something rarely found in the ancient DNA record.

Middle Bronze Age necropolis at Jagodnjak-Krčevine

The second site the researchers studied was the Middle Bronze Age necropolis of Jagodnjak-Krčevine that belongs to the Transdanubian Encrusted Pottery Culture and dates to around 1,800-1,600 BCE. "This site contains burials that are broadly contemporaneous with some individuals from the Dalmatian coast, and we wanted to find out whether individuals from these different ecoregions carried similar ancestry," says Stephan Schiffels.

The researchers found that the people from Jagodnjak actually carried very distinct ancestry due to the presence of significantly more western European hunter-gatherer-related ancestry. This ancestry profile is present in a small number of other studied genomes from further north in the Carpathian Basin. These new genetic results support archaeological evidence that suggests a shared population history for these groups as well as the presence of trade and exchange networks.

"We also found that all male individuals at the site had identical Y chromosome haplotypes," says Freilich. "We identified two male first degree relatives, second degree and more distantly related males, while the one woman in our sample was unrelated. This points to a patrilocal social organisation where women leave their own home to join their husband's home." Contrary to the Middle Neolithic site at Popova zemlja, biological kinship was a factor for selection to be buried at this site. In addition the authors found evidence of rich infant graves that suggests they likely inherited their status or wealth from their families.

Filling the gap in the archaeogenetic record

This study helps to fill the gap in the archaeogenetic record for this region, characterising the diverse genetic ancestries and social organisations that were present in Neolithic and Bronze Age eastern Croatia. It highlights the heterogeneous population histories of broadly contemporaneous coastal and inland Bronze Age groups, and connections with communities further north in the Carpathian Basin. Furthermore, it sheds light on the subject of Neolithic intramural burials -- burials within a settlement -- that has been debated among archaeologists and anthropologists for some time. The authors show that at the site of Popova zemlja, this burial rite was not associated with biological kinship, but more likely represented age and sex selection related to Neolithic community belief systems.

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