In Germany, there are more than 7,000 biogas plants which can supply over six million households with power. The plants are filled mostly with plant biomass like maize silage but also with agricultural waste materials like liquid manure and chicken manure. One of the key research questions is how the production of biogas can be optimised. For this reason, Bielefeld scientists Dr Alexander Sczyrba, Dr Andreas Schlüter, Dr Alexander Goesmann, Professor Dr Jens Stoye und Professor Dr Alfred Pühler want to know what microbes are responsible for the decomposition of biomass -- and which of them do it best. "We are interested in discovering the microbiology that is really behind the processes going on in a biogas plant; what micro-organisms play which role at which stage," explains Andreas Schlüter, whose research at CeBiTec is in the field of biogas production.
First genome deciphered
The researchers' work has already borne its first fruit. "At CeBiTec, we have managed to deci-pher the complete genome sequence of Methanoculleus bourgensis, a methane producer," reports Professor Pühler. By doing so, Bielefeld has sequenced the first genome for a methane-producing archaeon from a biogas plant -- a single-celled primordial bacterium which plays an important role in certain biogas plants. Now, the researchers want to go even further.
Putting the puzzle together
The project is part of the Community Sequencing Program, a public sequencing programme financed at the Joint Genome Institute by the US Department of Energy. While previous biogas studies have concentrated primarily on certain marker genes, now the entire genetic information of the microorganisms is to be studied. The American institute will produce more than one terabyte of sequence data for this, which is equivalent in volume to approximately 300 human genomes. This data will be supplied in a countless number of fragments, however, since even the most modern technology is not capable of reading all at once the millions of bases of which a microbial DNA molecule consists. Instead, the sequencing technologies supply vast quantities of overlapping sections of about 150 bases.
The DNA sequences will then be returned to Bielefeld in billions of fragments, which is where Alexander Sczyrba's Computa-tional Metagenomics team comes into play. They develop bioinformatic procedures for the reconstruction of genome sequences. Their task is to compare the data, recognise the overlaps and use them to reassemble the base sequence. "We are trying to complete a puzzle made up of billions of pieces, which also includes hundreds of different puzzles all mixed up," explains Sczyrba.
Single-cell genomics promises new insights
Quite incidentally, the Bielefeld researchers will be breaking new ground in genomics. An estimated 99 per cent of all microorganisms cannot be cultivated in the laboratory. A brand new technology, single-cell genomics, is to provide insights here by determining the genome sequence from single microbial cells. Knowledge of the identity and functions of hitherto completely unknown microorganisms is expected to be gained. During the joint project, the Joint Genome Institute will sequence approximately 100 single-cell genomes.
The researchers have scheduled roughly two years for their project, in which also Bielefeld doctoral students of the Graduate Cluster in Industrial Biotechnology (CLIB) are involved. At the end, they hope to have discovered the optimal microbial community for biogas plants -- and thus be in a position to make this process of generating energy even more efficient.
Read more at Sceince Daily
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