New technology designed to genetically control disease-spreading mosquitoes

Leveraging advancements in CRISPR-based genetic engineering, researchers at the University of California San Diego have created a new system that restrains populations of mosquitoes that infect millions each year with debilitating diseases.

The new precision-guided sterile insect technique, or pgSIT, alters genes linked to male fertility -- creating sterile offspring -- and female flight in Aedes aegypti, the mosquito species responsible for spreading wide-ranging diseases including dengue fever, chikungunya and Zika.

"pgSIT is a new scalable genetic control system that uses a CRISPR-based approach to engineer deployable mosquitoes that can suppress populations," said UC San Diego Biological Sciences Professor Omar Akbari. "Males don't transmit diseases so the idea is that as you release more and more sterile males, you can suppress the population without relying on harmful chemicals and insecticides."

Details of the new pgSIT are described September 10, 2021, in the journal Nature Communications.

pgSIT differs from "gene drive" systems that could suppress disease vectors by passing desired genetic alterations indefinitely from one generation to the next. Instead, pgSIT uses CRISPR to sterilize male mosquitoes and render female mosquitoes, which spread disease, as flightless. The system is self-limiting and is not predicted to persist or spread in the environment, two important safety features that should enable acceptance for this technology.

Akbari says the envisioned pgSIT system could be implemented by deploying eggs of sterile males and flightless females at target locations where mosquito-borne disease spread is occurring.

"Supported by mathematical models, we empirically demonstrate that released pgSIT males can compete, and suppress and even eliminate mosquito populations," the researchers note in the Nature Communications paper. "This platform technology could be used in the field, and adapted to many vectors, for controlling wild populations to curtail disease in a safe, confinable and reversible manner."

Although molecular genetic engineering tools are new, farmers have been sterilizing male insects to protect their crops since at least the 1930s. United States growers in the 1950s began using radiation to sterilize pest species such as the New World Screwworm fly, which is known to destroy livestock. Similar radiation-based methods continue today, along with the use of insecticides. pgSIT is designed as a much more precise and scalable technology since it uses CRISPR -- not radiation or chemicals -- to alter key mosquito genes. The system is based on a method that was announced by UC San Diego in 2019 by Akbari and his colleagues in the fruit fly Drosophila.

As envisioned, Akbari says pgSIT eggs can be shipped to a location threatened by mosquito-borne disease or developed at an on-site facility that could produce the eggs for nearby deployment. Once the pgSIT eggs are released in the wild, typically at a peak rate of 100-200 pgSIT eggs per Aedes aegypti adult, sterile pgSIT males will emerge and eventually mate with females, driving down the wild population as needed.

Beyond Aedes aegypti, the researchers believe the pgSIT technology could be directed to other species that spread disease.

"… This study suggests pgSIT may be an efficient technology for mosquito population control and the first example of one suited for real-world release," the researchers say. "Going forward, pgSIT may provide an efficient, safe, scalable, and environmentally friendly alternative next-generation technology for wild population control of mosquitoes resulting in wide-scale prevention of human disease transmission."

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Environmental conditions of early humans in Europe

Understanding the environmental conditions under which early humans dispersed out of Africa is important for understanding the factors that affected human evolution. This is a topical question that remains debated. A recent study prepared in collaboration with researchers from the University of Helsinki and the Universities of Granada, Tarragona, Zaragoza, Barcelona, Salamanca, Madrid and Tübingen provides new information on the environmental context of earliest human occupation in Europe during the Pleistocene.

The research is part of the Orce project (ProyectORCE) funded by the local government of Andalucía and led by the University of Granada, in which researchers from the University of Helsinki have been participating since 2017. The project is responsible for archeological/palaentological excavations and related research in Andalucía, Spain.

The study is focussed on the Guadix-Baza Basin, Andalucía, Spain, where the researchers used dental ecometric trait distribution within fossil large mammal communities to reconstruct climatic variables and net primary production of plant communities from ca. 4.5 million years to ca. 400,000 years ago.

The Guadix-Baza basin is of particular importance for understanding early human environments outside Africa, because it includes a couple of sites that are among the earliest human occupation sites in Europe, Barranco León and Fuente Nueva 3 near the city of Orce, which have been dated at ca. 1.4 -- 1.2 million years in age.

Based on the estimates, the climate in the Guadix-Baza Basin varied from roughly similar to present (e.g. Venta Micena, ca. 1.6 million years ago) to more humid, with higher annual primary production. The early human occupation sites in the Guadix-Baza Basin, such as Barranco León and Fuente Nueva 3, tended to have higher primary production than in the region today. The vegetation was mostly similar to Mediterranean forest without significant grassy undergrowth, making it different from African grass-dominated savanna environments.

Lead author Juha Saarinen from the University of Helsinki said: "Tooth wear-based dietary analyses indicate that most of the large herbivorous mammals in these environments did not consume significant amounts of grass, further attesting to the scarcity of grassy vegetation. This is an important finding, as it suggests that already the earliest human occupation sites in Europe were often different from African grassy savannas in terms of vegetation and interactions between large mammal fauna and vegetation."

The conditions under which early members of the genus Homo dispersed outside Africa were also analysed on a broader scale, across Europe during the Early and Middle Pleistocene. The model is based on the comparison of functional trait distribution of large herbivorous mammals in sites with archaeological or fossil evidence of human presence and in sites, which lack evidence of human presence.

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ESO captures best images yet of peculiar 'dog-bone' asteroid

Using the European Southern Observatory's Very Large Telescope (ESO's VLT), a team of astronomers have obtained the sharpest and most detailed images yet of the asteroid Kleopatra. The observations have allowed the team to constrain the 3D shape and mass of this peculiar asteroid, which resembles a dog bone, to a higher accuracy than ever before. Their research provides clues as to how this asteroid and the two moons that orbit it formed.

"Kleopatra is truly a unique body in our Solar System," says Franck Marchis, an astronomer at the SETI Institute in Mountain View, USA and at the Laboratoire d'Astrophysique de Marseille, France, who led a study on the asteroid -- which has moons and an unusual shape -- published today in Astronomy & Astrophysics. "Science makes a lot of progress thanks to the study of weird outliers. I think Kleopatra is one of those and understanding this complex, multiple asteroid system can help us learn more about our Solar System."

Kleopatra orbits the Sun in the Asteroid Belt between Mars and Jupiter. Astronomers have called it a "dog-bone asteroid" ever since radar observations around 20 years ago revealed it has two lobes connected by a thick "neck." In 2008, Marchis and his colleagues discovered that Kleopatra is orbited by two moons, named AlexHelios and CleoSelene, after the Egyptian queen's children.

To find out more about Kleopatra, Marchis and his team used snapshots of the asteroid taken at different times between 2017 and 2019 with the Spectro-Polarimetric High-contrast Exoplanet REsearch (SPHERE) instrument on ESO's VLT. As the asteroid was rotating, they were able to view it from different angles and to create the most accurate 3D models of its shape to date. They constrained the asteroid's dog-bone shape and its volume, finding one of the lobes to be larger than the other, and determined the length of the asteroid to be about 270 kilometres or about half the length of the English Channel.

In a second study, also published in Astronomy & Astrophysics and led by Miroslav Brož of Charles University in Prague, Czech Republic, the team reported how they used the SPHERE observations to find the correct orbits of Kleopatra's two moons. Previous studies had estimated the orbits, but the new observations with ESO's VLT showed that the moons were not where the older data predicted them to be.

"This had to be resolved," says Brož. "Because if the moons' orbits were wrong, everything was wrong, including the mass of Kleopatra." Thanks to the new observations and sophisticated modelling, the team managed to precisely describe how Kleopatra's gravity influences the moons' movements and to determine the complex orbits of AlexHelios and CleoSelene. This allowed them to calculate the asteroid's mass, finding it to be 35% lower than previous estimates.

Combining the new estimates for volume and mass, astronomers were able to calculate a new value for the density of the asteroid, which, at less than half the density of iron, turned out to be lower than previously thought. The low density of Kleopatra, which is believed to have a metallic composition, suggests that it has a porous structure and could be little more than a "pile of rubble." This means it likely formed when material reaccumulated following a giant impact.

Kleopatra's rubble-pile structure and the way it rotates also give indications as to how its two moons could have formed. The asteroid rotates almost at a critical speed, the speed above which it would start to fall apart, and even small impacts may lift pebbles off its surface. Marchis and his team believe that those pebbles could subsequently have formed AlexHelios and CleoSelene, meaning that Kleopatra has truly birthed its own moons.

The new images of Kleopatra and the insights they provide are only possible thanks to one of the advanced adaptive optics systems in use on ESO's VLT, which is located in the Atacama Desert in Chile. Adaptive optics help to correct for distortions caused by the Earth's atmosphere which cause objects to appear blurred -- the same effect that causes stars viewed from Earth to twinkle. Thanks to such corrections, SPHERE was able to image Kleopatra -- located 200 million kilometres away from Earth at its closest -- even though its apparent size on the sky is equivalent to that of a golf ball about 40 kilometres away.

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Surprise: The Milky Way is not homogeneous

In order to better understand the history and evolution of the Milky Way, astronomers are studying the composition of the gases and metals that make up an important part of our galaxy. Three main elements stand out: the initial gas coming from outside our galaxy, the gas between the stars inside our galaxy -- enriched with chemical elements -, and the dust created by the condensation of the metals present in this gas. Until now, theoretical models assumed that these three elements were homogeneously mixed throughout the Milky Way and reached a level of chemical enrichment similar to the Sun's atmosphere, called the Solar metallicity. Today, a team of astronomers from the University of Geneva (UNIGE) demonstrates that these gases are not mixed as much as previously thought, which has a strong impact on the current understanding of the evolution of galaxies. As a result, simulations of the Milky Way's evolution will have to be modified. These results can be read in the journal Nature.

Galaxies are made up of a collection of stars and are formed by the condensation of the gas of the intergalactic medium composed of mostly hydrogen and a bit of helium. This gas does not contain metals unlike the gas in galaxies -- in astronomy, all chemical elements heavier than helium are collectively called "metals," although they are atoms in gaseous form. "Galaxies are fuelled by 'virgin' gas that falls in from the outside, which rejuvenates them and allows new stars to form," explains Annalisa De Cia, a professor in the Department of Astronomy at the UNIGE Faculty of Science and first author of the study. At the same time, stars burn the hydrogen that constitutes them throughout their life and form other elements through nucleosynthesis. When a star that has reached the end of its life explodes, it expels the metals it has produced, such as iron, zinc, carbon and silicon, feeding these elements into the gas of the galaxy. These atoms can then condense into dust, especially in the colder, denser parts of the galaxy. "Initially, when the Milky Way was formed, more than 10 billion years ago, it had no metals. Then the stars gradually enriched the environment with the metals they produced," continues the researcher. When the amount of metals in this gas reaches the level that is present in the Sun, astronomers speak of Solar metallicity.

A not so homogeneous environment

The environment that makes up the Milky Way thus brings together the metals produced by the stars, the dust particles that have formed from these metals, but also gases from outside the galaxy that regularly enter it. "Until now, theoretical models considered that these three elements were homogeneously mixed and reached the Solar composition everywhere in our galaxy, with a slight increase in metallicity in the centre, where the stars are more numerous," explains Patrick Petitjean, a researcher at the Institut d'Astrophysique de Paris, Sorbonne University. "We wanted to observe this in detail using an Ultraviolet spectrograph on the Hubble Space Telescope."

Spectroscopy allows the light from stars to be separated in its individual colors or frequencies, a bit like a with prism or in a rainbow. In this decomposed light, astronomers are particularly interested in absorption lines: "When we observe a star, the metals that make up the gas between the star and ourselves absorb a very small part of the light in a characteristic way, at a specific frequency, which allows us not only to identify their presence, but also to say which metal it is, and how abundant it is," he continues.

A new method developed to observe the total metallicity

For 25 hours, the team of scientists observed the atmosphere of 25 stars using Hubble and the Very Large Telescope (VLT) in Chile. The problem? The dust cannot be counted with these spectrographs, even though it contains metals. Annalisa De Cia's team has therefore developed a new observational technique. "It involves taking into account the total composition of the gas and dust by simultaneously observing several elements such as iron, zinc, titanium, silicon and oxygen," explains the Geneva researcher. "Then we can trace the quantity of metals present in the dust and add it to that already quantified by the previous observations to get the total."

Thanks to this dual observation technique, the astronomers have found that not only is the Milky Way's environment not homogeneous, but that some of the areas studied reach only 10% of the Solar metallicity. "This discovery plays a key role in the design of theoretical models on the formation and evolution of galaxies," says Jens-Kristian Krogager, researcher at the UNIGE's Department of Astronomy. "From now on, we will have to refine the simulations by increasing the resolution, so that we can include these changes in metallicity at different locations in the Milky Way."

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Too much free time may be almost as bad as too little

As an individual's free time increases, so does that person's sense of well-being -- but only up to a point. Too much free time can be also be a bad thing, according to research published by the American Psychological Association.

"People often complain about being too busy and express wanting more time. But is more time actually linked to greater happiness? We found that having a dearth of discretionary hours in one's day results in greater stress and lower subjective well-being," said Marissa Sharif, PhD, an assistant professor of marketing at The Wharton School and lead author of the paper. "However, while too little time is bad, having more time is not always better."

The research was published in the Journal of Personality and Social Psychology.

Researchers analyzed the data from 21,736 Americans who participated in the American Time Use Survey between 2012 and 2013. Participants provided a detailed account of what they did during the prior 24 hours -- indicating the time of day and duration of each activity -- and reported their sense of well-being. The researchers found that as free time increased, so did well-being, but it leveled off at about two hours and began to decline after five. Correlations in both directions were statistically significant.

The researchers also analyzed data from 13,639 working Americans who participated in the National Study of the Changing Workforce between 1992 and 2008. Among the survey's many questions, participants were asked about their amount of discretionary time (e.g., "On average, on days when you're working, about how many hours [minutes] do you spend on your own free-time activities?") and their subjective well-being, which was measured as life satisfaction (e.g., "All things considered, how do you feel about your life these days? Would you say you feel 1=very satisfied, 2=somewhat satisfied, 3=somewhat dissatisfied, or 4=very dissatisfied?")

Once again, the researchers found that higher levels of free time were significantly associated with higher levels of well-being, but only up to a point. After that, excess free time was not associated with greater well-being.

To further investigate the phenomenon, the researchers conducted two online experiments involving more than 6,000 participants. In the first experiment, participants were asked to imagine having a given amount of discretionary time every day for at least six months. Participants were randomly assigned to have a low (15 minutes per day), moderate (3.5 hours per day), or high (7 hours per day) amount of discretionary time. Participants were asked to report the extent to which they would experience enjoyment, happiness and satisfaction.

Participants in both the low and high discretionary time groups reported lower well-being than the moderate discretionary time group. The researchers found that those with low discretionary time felt more stressed than those with a moderate amount, contributing to lower well-being, but those with high levels of free time felt less productive than those in the moderate group, leading them to also have lower well-being.

In the second experiment, researchers looked at the potential role of productivity. Participants were asked to imagine having either a moderate (3.5 hours) or high (7 hours) amount of free time per day, but were also asked to imagine spending that time in either productive (e.g., working out, hobbies or running) or unproductive activities (e.g., watching television or using the computer). The researchers found participants with more free time reported lower levels of well-being when engaging in unproductive activities. However, when engaging in productive activities, those with more free time felt similar to those with a moderate amount of free time.

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Steps per day matter in middle age, but not as many as you may think

Walking at least 7,000 steps a day reduced middle-aged people's risk of premature death from all causes by 50% to 70%, compared to that of other middle-aged people who took fewer daily steps.

But walking more than 10,000 steps per day -- or walking faster -- did not further reduce the risk, notes lead author Amanda Paluch, a physical activity epidemiologist at the University of Massachusetts Amherst.

The findings, published in JAMA Network Open, highlight the evolving efforts to establish evidence-based guidelines for simple, accessible physical activity that benefits health and longevity, such as walking. The oft-advised 10,000 steps a day is not a scientifically established guideline but emerged as part of a decades-old marketing campaign for a Japanese pedometer, says Paluch, assistant professor of kinesiology in the School of Public Health and Health Sciences.

One question Paluch and colleagues wanted to begin to answer: How many steps per day do we need for health benefits? "That would be great to know for a public health message or for clinician-patient communication," she says.

The researchers mined data from the Coronary Artery Risk Development in Young Adults (CARDIA) study, which began in 1985 and is still ongoing. Some 2,100 participants between age 38 and 50 wore an accelerometer in 2005 or 2006. They were followed for nearly 11 years after that, and the resulting data were analyzed in 2020 and 2021.

The participants were separated into three comparison groups: low-step volume (under 7,000 per day), moderate (between 7,000-9,999) and high (more than 10,000).

"You see this gradual risk reduction in mortality as you get more steps," Paluch says. "There were substantial health benefits between 7,000 and 10,000 steps but we didn't see an additional benefit from going beyond 10,000 steps.

"For people at 4,000 steps, getting to 5,000 is meaningful," she adds. "And from 5,000 to 6,000 steps, there is an incremental risk reduction in all-cause mortality up to about 10,000 steps."

Several features make this study particularly interesting and informative. For one, it involved people in middle age, while most step studies have been focused on older adults. So the findings can begin to suggest ways to keep people healthier longer and to avoid premature death, as some of the participants experienced.

"Preventing those deaths before average life expectancy -- that is a big deal," Paluch says. "Showing that steps per day could be associated with premature mortality is a new contribution to the field."

The study also featured an equal number of men and women and Black and white participants. Death rates for people walking at least 7,000 steps per day were lowest among women and Blacks, compared to their more sedentary peers. But there was a limited sample of people who died, and Paluch cautions that researchers need to study larger diverse populations to gauge statistically significant sex and race differences.

Paluch is eager to continue researching the impact of steps per day on health and how walking may be beneficial in a variety of ways at different life stages.

Read more at Science Daily

Preventing the long-term effects of traumatic brain injury

You've been in a car accident and sustained a head injury. You recovered, but years later you begin having difficulty sleeping. You also become very sensitive to noise and bright lights, and find it hard to carry out your daily activities, or perform well at your job.

This is a common situation after a traumatic brain injury -- many people experience bad side effects months or years later. These long-term effects can last a few days or the rest of a person's life.

"No therapies currently exist to prevent the disabilities that can develop after a brain trauma," says Jeanne Paz, PhD, associate investigator at Gladstone Institutes. "So, understanding how the traumatic brain injury affects the brain, especially in the long term, is a really important gap in research that could help develop new and better treatment options."

In a new study published in the journal Science, Paz and her team helped close that gap. They identified a specific molecule in a part of the brain called the thalamus that plays a key role in secondary effects of brain injury, such as sleep disruption, epileptic activity, and inflammation. In collaboration with scientists at Annexon Biosciences, a clinical-stage biopharmaceutical company, they also showed that an antibody treatment could prevent the development of these negative outcomes.

A Vulnerable Brain Region

Traumatic brain injuries, which range from a mild concussion to a severe injury, can be the result of a fall, sports injury, gunshot injury, blow to the head, explosion, or domestic violence. Often, soldiers returning from war also suffer head injuries, which commonly lead to the development of epilepsy. Traumatic brain injury affects 69 million people around the world annually, and is the leading cause of death in children and a major source of disability in adults.

"These injuries are frequent and can happen to anyone," says Paz, who is also an associate professor of neurology at UC San Francisco (UCSF) and a member of the Kavli Institute for Fundamental Neuroscience. "The goal of our study was to understand how the brain changes after traumatic brain injuries and how those changes can lead to chronic problems, such as the development of epilepsy, sleep disruption, and difficulty with sensory processing."

To do so, Paz and her team recorded the activity of different cells and circuits in the brain of mice after brain injury. The researchers monitored the mice continually and wirelessly, meaning the mice could go about their normal activities without being disrupted.

"We collected so much data, from the time of injury and over the next several months, that it actually crashed our computers," says Paz. "But it was important to capture all the different stages of sleep and wakefulness to get the whole picture."

During a trauma to the head, the region of the brain called the cerebral cortex is often the primary site of injury, because it sits directly beneath the skull.

But at later time points, the researchers discovered that another region -- the thalamus -- was even more disrupted than the cortex. In particular, they found that a molecule called C1q was present at abnormally high levels in the thalamus for months after the initial injury, and these high levels were associated with inflammation, dysfunctional brain circuits, and the death of neurons.

"The thalamus seems particularly vulnerable, even after a mild traumatic brain injury," says Stephanie Holden, PhD, first author of the study and former graduate student in Paz's lab at Gladstone. "This doesn't mean the cortex isn't affected, but simply that it might have the necessary tools to recover over time. Our findings suggest that the higher levels of C1q in the thalamus could contribute to several long-term effects of brain injury."

The Paz Lab collaborated with Eleonora Aronica, MD, PhD, a neuropathologist at the University of Amsterdam, to validate their findings in human brain tissues obtained from autopsies, in which they found high levels of the C1q molecule in the thalamus 8 days after people had sustained a traumatic brain injury. In addition, by working with fellow Gladstone Assistant Investigator Ryan Corces, PhD, they determined that C1q in the thalamus likely came from microglia, the immune cells in the brain.

"Our study answered some very big questions in the field about where and how changes are happening in the brain after a trauma, and which ones are actually important for causing deficits," says Paz.

The Right Window to Treat Chronic Effects After Traumatic Brain Injury

The C1q molecule, which is part of an immune pathway, has well-documented roles in brain development and normal brain functions. For instance, it protects the central nervous system from infection and helps the brain forget memories -- a process needed to store new memories. The accumulation of C1q in the brain has also been studied in various neurological and psychiatric disorders and is associated, for example, with Alzheimer's disease and schizophrenia.

"C1q can be both good and bad," says Paz. "We wanted to find a way to prevent this molecule's detrimental effect, but without impacting its beneficial role. This is an example of what makes neuroscience a really hard field these days, but it's also what makes it exciting."

She and her group decided to leverage the "latent phase" after a traumatic brain injury, during which changes are occurring in the brain but before long-term symptoms appear.

"My cousin, for example, was hit in the head when he was 10 years old, and the impact broke his skull and damaged his brain," says Paz. "But it wasn't until he was 20 that he developed epilepsy. This latent phase presents a window of opportunity for us to intervene in hopes of modifying the disease and preventing any complications."

Paz reached out to her collaborators at Annexon Biosciences, who produce a clinical antibody that can block the activity of the C1q molecule. Then, her team treated the mice who sustained brain injury with this antibody to see if it might have beneficial effects.

When the researchers studied mice genetically engineered to lack C1q at the time of the trauma, the brain injury appeared much worse. However, when they selectively blocked C1q with the antibody during the latent phase, they prevented chronic inflammation and the loss of neurons in the thalamus.

"This indicates that the C1q molecule shouldn't be blocked at the time of injury, because it's likely very important at this stage for protecting the brain and helping prevent cell death," says Holden. "But at later time points, blocking C1q can actually reduce harmful inflammatory responses. It's a way of telling the brain, 'It's okay, you've done the protective part and you can now turn off the inflammation.'"

"There is a paucity of treatments for patients who have suffered from an acute brain injury," says Ted Yednock, PhD, executive vice president and chief scientific officer at Annexon Biosciences, and an author of the study. "This result is exciting because it suggests that we could treat patients in the hours to days after an acute injury like traumatic brain injury to protect against secondary neuronal damage and provide significant functional benefit."

Path to a Potential Treatment

In addition to chronic inflammation, Paz and her team also uncovered abnormal brain activity in the mice with traumatic brain injury.

First, the researchers noticed disruptions in sleep spindles, which are normal brain rhythms that occur during sleep. These are important for memory consolidation, among other things. The scientists also found epileptic spikes, or abnormal fluctuations in brain activity. These spikes can be disruptive to cognition and normal behavior, and are also indicative of a greater susceptibility to seizures.

The scientists observed that the anti-C1q antibody treatment not only helped restore the sleep spindles, but also prevented the development of epileptic activities.

"Overall, our study indicates that targeting the C1q molecule after injury could avoid some of the most devastating, long-term consequences of traumatic brain injury," says Holden. "We hope this could eventually lead to the development of treatments for traumatic brain injury."

Annexon's anti-C1q inhibitors are designed to treat multiple autoimmune and neurological disorders, and are already being examined in clinical trials, including for an autoimmune disorder known as Guillain-Barré syndrome, where the drug has been shown to be safe in humans.

"The fact that the drug is already in clinical trials may speed the pace at which a treatment could eventually be made available to patients," says Yednock. "We already understand doses of drug that are safe and effective in patients for blocking C1q in the brain, and could move directly into studies that ameliorate the chronic effects after traumatic brain injury."

For Holden, who previously worked with individuals who experienced brain injury and heard many of their personal stories, the impact of this study is particularly meaningful.

"Brain injury is a hidden disability for many of the people I met," she says. "The side effects they experience can be difficult to diagnose and their physicians often can't provide any medical treatment. Being able to contribute to finding ways to treat the detrimental consequences of the injury after it happens is really inspiring."

Paz and her lab are continuing to expand their understanding of what happens in the brain after injury. Next, they will focus on studying whether they can help prevent convulsive seizures, which are often reported by people with severe traumatic brain injuries.

Read more at Science Daily

How land birds cross the open ocean

Researchers at the Max Planck Institute of Animal Behavior and University of Konstanz in Germany have identified how large land birds fly nonstop for hundreds of kilometers over the open ocean—without taking a break for food or rest. Using GPS tracking technology, the team monitored the global migration of five species of large land birds that complete long sea crossings. They found that all birds exploited wind and uplift to reduce energy costs during flight—even adjusting their migratory routes to benefit from the best atmospheric conditions. This is the most wide-ranging study of sea-crossing behavior yet and reveals the important role of the atmosphere in facilitating migration over the open sea for many terrestrial birds.

Flying over the open sea can be dangerous for land birds. Unlike seabirds, land birds are not able to rest or feed on water, and so sea crossings must be conducted as nonstop flights. For centuries, bird-watchers assumed that large land birds only managed short sea crossings of less than 100 kilometers and completely avoided flying over the open ocean.

However, recent advances in GPS tracking technology have overturned that assumption. Data obtained by attaching small tracking devices on wild birds has shown that many land birds fly for hundreds or even thousands of kilometers over the open seas and oceans as a regular part of their migration.

But scientists are still unraveling how land birds are able to accomplish this. Flapping is an energetically costly activity, and trying to sustain nonstop flapping flight for hundreds of kilometers would not be possible for large, heavy land birds. Some studies have suggested that birds sustain such journeys using tailwind, a horizontal wind blowing in the bird’s direction of flight, which helps them save energy. Most recently, a study revealed that a single species—the osprey—used rising air thermals known as “uplift” to soar over the open sea.

Now, the new study has examined sea-crossing behavior of 65 birds across five species to gain the most wide-ranging insight yet into how land birds survive long flights over the open sea. The researchers analyzed 112 sea-crossing tracks, collected over nine years, with global atmospheric information to pinpoint the criteria that the birds use for selecting their migration routes over the open sea. A large international collaboration of scientists shared their tracking data to make this study possible.

The findings not only confirm the role of tailwind in facilitating sea-crossing behavior, but also reveal the widespread use of uplift for saving energy during these nonstop flights. Suitable uplift means less drag, making sea crossing less energetically demanding.

“Until recently, uplift was assumed to be weak or absent over the sea surface. We show that is not the case,” says first author Elham Nourani, a DAAD PRIME postdoctoral fellow at the Department of Biology at the University of Konstanz, who did the work when she was at the Max Planck Institute of Animal Behavior.

“Instead, we find that migratory birds adjust their flight routes to benefit from the best wind and uplift conditions when they fly over the sea. This helps them sustain flight for hundreds of kilometers,” says Nourani.

The oriental honey buzzard, for example, flies 700 kilometers over the East China Sea during its annual migration from Japan to southeast Asia. The roughly 18-hour nonstop sea crossing is conducted in autumn when the air movement conditions are optimal. “By making use of uplift, these birds can soar up to one kilometer above the sea surface,” says Nourani.

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Massive new animal species discovered in half-billion-year-old Burgess Shale

Palaeontologists at the Royal Ontario Museum (ROM) have uncovered the remains of a huge new fossil species belonging to an extinct animal group in half-a-billion-year-old Cambrian rocks from Kootenay National Park in the Canadian Rockies. The findings were announced on September 8, 2021, in a study published in Royal Society Open Science.

Named Titanokorys gainesi, this new species is remarkable for its size. With an estimated total length of half a meter, Titanokorys was a giant compared to most animals that lived in the seas at that time, most of which barely reached the size of a pinky finger.

"The sheer size of this animal is absolutely mind-boggling, this is one of the biggest animals from the Cambrian period ever found," says Jean-Bernard Caron, ROM's Richard M. Ivey Curator of Invertebrate Palaeontology.

Evolutionarily speaking, Titanokorys belongs to a group of primitive arthropods called radiodonts. The most iconic representative of this group is the streamlined predator Anomalocaris, which may itself have approached a metre in length. Like all radiodonts, Titanokorys had multifaceted eyes, a pineapple slice-shaped, tooth-lined mouth, a pair of spiny claws below its head to capture prey and a body with a series of flaps for swimming. Within this group, some species also possessed large, conspicuous head carapaces, with Titanokorys being one of the largest ever known.

"Titanokorys is part of a subgroup of radiodonts, called hurdiids, characterized by an incredibly long head covered by a three-part carapace that took on myriad shapes. The head is so long relative to the body that these animals are really little more than swimming heads," added Joe Moysiuk, co-author of the study, and a ROM-based Ph.D. student in Ecology & Evolutionary Biology at the University of Toronto.

Why some radiodonts evolved such a bewildering array of head carapace shapes and sizes is still poorly understood and was likely driven by a variety of factors, but the broad flattened carapace form in Titanokorys suggests this species was adapted to life near the seafloor.

"These enigmatic animals certainly had a big impact on Cambrian seafloor ecosystems. Their limbs at the front looked like multiple stacked rakes and would have been very efficient at bringing anything they captured in their tiny spines towards the mouth. The huge dorsal carapace might have functioned like a plough," added Dr. Caron, who is also an Associate Professor in Ecology & Evolutionary Biology and Earth Sciences at the University of Toronto, and Moysiuk's Ph.D. advisor.

All fossils in this study were collected around Marble Canyon in northern Kootenay National Park by successive ROM expeditions. Discovered less than a decade ago, this area has yielded a great variety of Burgess Shale animals dating back to the Cambrian period, including a smaller, more abundant relative of Titanokorys named Cambroraster falcatusin reference to its Millennium Falcon-shaped head carapace. According to the authors, the two species might have competed for similar bottom-dwelling prey.

The Burgess Shale fossil sites are located within Yoho and Kootenay National Parks and are managed by Parks Canada. Parks Canada is proud to work with leading scientific researchers to expand knowledge and understanding of this key period of earth history and to share these sites with the world through award-winning guided hikes. The Burgess Shale was designated a UNESCO World Heritage Site in 1980 due to its outstanding universal value and is now part of the larger Canadian Rocky Mountain Parks World Heritage Site.

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Who was king before Tyrannosaurus? Uzbek fossil reveals new top dino

Iconic tyrannosauroids like T. rex famously dominated the top of the food web at the end of the reign of the dinosaurs. But they didn't always hold that top spot.

In a new study published in Royal Society Open Science, a research team led by the University of Tsukuba has described a new genus and species belonging to the Carcharodontosauria, a group of medium- to large-sized carnivorous dinosaurs that preceded the tyrannosauroids as apex predators.

The new dinosaur, named Ulughbegsaurus uzbekistanensis, was found in the lower Upper Cretaceous Bissekty Formation of the Kyzylkum Desert in Uzbekistan, and therefore lived about 90 million years ago. Two separate evolutionary analyses support classification of the new dinosaur as the first definitive carcharodontosaurian discovered in the Upper Cretaceous of Central Asia.

"We described this new genus and species based on a single isolated fossil, a left maxilla, or upper jawbone," explains study first author Assistant Professor Kohei Tanaka. "Among theropod dinosaurs, the size of the maxilla can be used to estimate the animal's size because it correlates with femur length, a well-established indicator of body size. Thus, we were able to estimate that Ulughbegsaurus uzbekistanensis had a mass of over 1,000 kg, and was approximately 7.5 to 8.0 meters in length, greater than the length of a full-grown African elephant."

This size greatly exceeds that of any other carnivore known from the Bissekty Formation, including the small-sized tyrannosauroid Timurlengia described from the same formation. Therefore, the newly named dinosaur likely topped the food web in its early Late Cretaceous ecosystem.

The genus's namesake is fittingly regal; Ulughbegsaurus is named for Ulugh Beg, the 15th century mathematician, astronomer, and sultan of the Timurid Empire of Central Asia. The species is named for the country where the fossil was discovered.

Before the Late Cretaceous, carcharodontosaurians like Ulughbegsaurus disappeared from the paleocontinent that included Central Asia. This disappearance is thought to have been related to the rise of tyrannosauroids as apex predators, but this transition has remained poorly understood because of the scarcity of relevant fossils.

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