NC State developmental biologist Nanette Nascone-Yoder, graduate student Stephanie Bloom and postdoc Cris Ledon-Rettig looked at Xenopus laevis (African clawed frog) and Lepidobatrachus laevis (Budgett's frog) tadpoles. These frog species differ in diet and last shared a common ancestor about 110 million years ago. Like most tadpoles, Xenopus exist primarily on a diet of algae, and their long, simple digestive tracts are not able to process insects or proteins until they become adult frogs. Budgett's is an aggressive species of frog which is carnivorous -- and cannibalistic -- in the tadpole stage.
Nascone-Yoder knew that Budgett's tadpoles had evolved shorter, more complex guts to digest protein much earlier in their development. She and her team exposed Xenopus embryos to molecules that inactivated a variety of genes to see if any might coax Xenopus to develop a more carnivore-like digestive tract. Remarkably, five molecules caused Xenopus tadpoles to develop guts that were closer in appearance to those of the Budgett's tadpoles. Taking it one step further, Nascone-Yoder exposed Budgett's frog embryos to molecules with opposite effects, and got tadpole guts that were closer to those of Xenopus.
"Essentially, these molecules are allowing us to tease apart the processes that play a key role in gut development," Nascone-Yoder says. "Understanding how and why the gut develops different shapes and lengths to adapt to different diets and environments during evolution gives us insight into what types of processes can be altered in the context of human birth defects, another scenario in which the gut also changes its shape and function."
The researchers' next steps include finding out whether the changes in these gut tubes were merely cosmetic, or if they also function (digest) differently.
Read more at Science Daily
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